Simple, Precise, Accurate and Rapid methods for simultaneous estimation of Cinitapride Hydrogen Tartrate (CNT) and Omeprazole (OMP) in combined capsule dosage form have been developed. Method A is based on Ratio Spectra Derivative and Method B uses integrated area under curve (AUC), Methanol is used as a solvent for both the methods. In Method A, the amplitudes at 251 nm and 294 nm of the first derivative of ratio spectra were selected to determine CNT and OMP respectively by ratio derivative method. In Method B, the wavelength ranges of 260 – 270 nm and 296 – 306 nm were selected to determine CNT and OMP by AUC method in combined formulation. The linearity was found to be 1.5 - 4.5 µg/ml and 10 – 30 µg/ml for CNT and OMP respectively by both the methods. The % assay for CNT and OMP were found to be 99.66% and 99.89% by Method A & 100.83% and 99.74% by Method B respectively. The methods were validated with respect to linearity, precision and accuracy. The Average Percentage Mean Recovery was found to be 99.24% for CNT and 99.28% for OMP by ratio derivative method and 101.20% for CNT and 99.30% for OMP by AUC method. The developed methods are simple, economical, precise and accurate and can be used for routine quality control of analytes in combined formulations.
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